Sravnenie onkoliticheskoj aktivnosti rekombinantnyh shtammov virusa ospovakciny LIVP-RFP i MVA-RFP v otnoshenii solidnyh opuholej
نویسندگان
چکیده
Sredi onkoliticheskih virusov odnim iz naibolee izuchennyh yavlyaetsya virus ospovakciny (VV), shtamma modificirovannogo vysokoattenuirovannogo virusa Ankara (MVA), pokazavshego mnogoobeshchayushchie rezul'taty v doklinicheskih i klinicheskih ispytaniyah. SHtamm Lister VV Moskovskogo Instituta virusnyh preparatov (LIVP) issledovan men'shej stepeni, chem MVA imeet otlichnyj ot tropizm. Cel'yu raboty bylo sravnit' onkoliticheskuyu effektivnost' shtammov LIVP otnoshenii solidnyh opuholej. Dlya povysheniya selektivnosti k opuholevym kletkam nami byli polucheny rekombinantnye varianty s inaktivaciej gena timidinkinazy (TK), MVA-RFP LIVP-RFP, ekspressiruyushchie krasnyj fluorescentnyj belok. Kinetiku replikacii aktivnost' poluchennyh rekombinantnyh ocenivali in vitro vivo na liniyah opuholevyh kletok allotransplantatah myshinyh singennyh modelej metastaticheskoj adenokarcinomy molochnoj zhelezy myshi 4T1, tolstoj kishki CT26 melanomy B16. Kak MVA-RFP, tak LIVP-RFP pokazali vysokuyu kletkah vyrazhennuyu allotransplantatov V16 4T1. V yavlyayushchejsya model'yu trojnogo negativnogo raka cheloveka, po sravneniyu pokazal bolee 50% povyshennuyu citotoksichnost' testah vitro, a takzhe dostovernoe zamedlenie progressirovaniya 4T1 povyshenie vyzhivaemosti zhivotnyh eksperimentah vivo. Primenenie kachestve platformy pri razrabotke dlya terapii mozhet byt' perspektivnym, primenenie MVA.
منابع مشابه
DNA mimics of red fluorescent proteins (RFP) based on G-quadruplex-confined synthetic RFP chromophores
Red fluorescent proteins (RFPs) have emerged as valuable biological markers for biomolecule imaging in living systems. Developing artificial fluorogenic systems that mimic RFPs remains an unmet challenge. Here, we describe the design and synthesis of six new chromophores analogous to the chromophores in RFPs. We demonstrate, for the first time, that encapsulating RFP chromophore analogues in ca...
متن کاملAn Agile Request For Proposal (RFP) Process
The Request For Proposal (RFP) process can be agile and efficient. At a high level, the key to achieving this is to specify requirements just in time and containing just enough detail. This paper applies the following XP practices and concepts to the RFP process: acceptance tests, business value, iterative & incremental delivery, onsite customer, pair development, planning game, spike, story, v...
متن کاملThe plasma boundary in Single Helical Axis RFP plasmas
Single Helical Axis (SHAx) states obtained in high current reversed field pinch (RFP) plasmas display, aside from a dominant mode in the m=1 spectrum, also a dominant m=0 mode, with the same toroidal mode number as the m=1 one. The two modes have a fixed phase relationship. The island chain created by the m=0 mode across the reversal surface gives rise, at shallow reversal of the toroidal field...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Vestnik RGMU
سال: 2023
ISSN: ['2070-7320', '2070-7339']
DOI: https://doi.org/10.24075/vrgmu.2023.010